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Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC <t>phalloidine.</t> Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).
Fluorescein Phalloidin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ifluor 488 conjugated phalloidin  (Yeasen Biotechnology)
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Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC <t>phalloidine.</t> Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).
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fitc labeled phalloidin  (Beijing Solarbio Science)
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Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC <t>phalloidine.</t> Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).
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488 phalloidin  (Thermo Fisher)
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Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC <t>phalloidine.</t> Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).
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fitc phalloidin dye  (Yeasen Biotechnology)
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Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC <t>phalloidine.</t> Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).
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Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC <t>phalloidine.</t> Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).
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phalloidin fitc  (Yeasen Biotechnology)
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Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC <t>phalloidine.</t> Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).
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Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC phalloidine. Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).

Journal: Bioactive Materials

Article Title: A safe and anti-inflammatory plant-derived nanovesicle platform for targeted delivery in acute lung injury

doi: 10.1016/j.bioactmat.2026.03.033

Figure Lengend Snippet: Good biocompatibility of GRb1@LEVs-cRGD in vitro and in vivo . (a-c) CCK-8 assays in RAW264.7, MLE-12 and HUVEC cells, respectively (n = 3). (d) Live/dead cell staining in RAW264.7, MLE-12 and HUVEC cells. Green, Calcein AM staining for live cells. Red, propidium iodide staining for dead cells (n = 3). (e) Cell skeleton staining in HUVECs. Green, FITC phalloidine. Blue, DAPI. (f) Blood routine and biochemical indexes ( n = 3). (g) Representative TUNEL staining of lung tissues from GRb1@LEVs-cRGD and control groups (n = 3). (h) Histopathological examination by H&E staining of major organs (n = 3).

Article Snippet: Fluorescein phalloidin and cyanine5.5 amine were procured from MedChemExpress (USA).

Techniques: In Vitro, In Vivo, CCK-8 Assay, Staining, TUNEL Assay, Control